anti-cd137 (17b5  (Thermo Fisher)

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: CD137 expression correlated with intrarenal lymphangiogenesis and fibrosis in IgAN. (A) IHC analysis of CD137 expression (40×); Masson's trichome staining showing collagen deposition(40×); (B) Representative images and double-positive analyses for immunofluorescence-labeled D2-40 (green) and CD137 (red) in IgA patients (n=85) and controls (n=5). Scale bar, 20µm. (C) Relationships between CD137 + lymphatic vessels in the interstitium and Serum urea nitrogen, Serum Creatinine, Serum uric acid, proteinuria, Plasma albumin, HB and eGFR. HB, hemoglobin; eGFR, estimated glomerular filtration rate; (D) The difference of density of lymphatic vessels and CD137 + lymphatic vessels between patients with different T and S grades. T, tubular atrophy/interstitial fibrosis; S, segmental glomerulosclerosis.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Expressing, Staining, Immunofluorescence, Labeling, Clinical Proteomics, Filtration

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: Demographic and clinical parameters between patients with D2-40 and CD137 + D2-40 expression.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Expressing, Clinical Proteomics

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: Histopathologic features from renal biopsies between patients with low or high levels of CD137 + D2-40 expression.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Expressing

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: CD137 is increased in the obstructed kidney. (A) IHC staining showing LYVE-1 and CD137 expression in the sham control and UUO mice (400×). The expression levels of Prox-1 and LYVE-1 were detected by Western blot (B), and the expression levels of CD137 were detected by real-time PCR (C). (D) Immunofluorescence staining showing CD137 (red) expression in UUO kidneys in the interstitium on days 7 and 14 and colocalization with LYVE-1 (green) original magnification, ×400. n = 6 per group. The error bars represent the SEM. ***P < 0.001.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Immunohistochemistry, Expressing, Control, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

Journal: International Journal of Biological Sciences

Article Title: CD137L-macrophage induce lymphatic endothelial cells autophagy to promote lymphangiogenesis in renal fibrosis

doi: 10.7150/ijbs.66781

Figure Lengend Snippet: Demographic and clinical parameters between patients with low or high levels of CD137 + D2-40 expression.

Article Snippet: The renal fibrosis model was induced by unilateral ureteral obstruction (UUO) as previously reported. anti-CD137 blocking antibody (4-1BB, Clone:17B5, Bio X Cell, Lebanon NH) were injected (i.p.) at 10 mg/kg/dose twice weekly.

Techniques: Expressing, Clinical Proteomics

Journal: eLife

Article Title: SLAMF6​ deficiency augments tumor killing and skews toward an effector phenotype revealing it as a novel T cell checkpoint

doi: 10.7554/eLife.52539

Figure Lengend Snippet: ( A ) SLAMF6 and Vβ13 expression in Pmel-1 or Pmel-1 x SLAMF6 -/- splenocytes measured by flow cytometry. ( B ) Percent CD8+, CD4, and CD19 cells in spleens from Pmel-1 or Pmel-1 x SLAMF6 -/- untreated mice. ( C ) Pmel-1, and Pmel-1 x SLAMF6 -/- CD8+ untreated splenocytes were stained with anti-CD44 and anti-CD62L. One representative experiment is shown. ( D ) Percent CD8+ cells in Pmel-1 or Pmel-1 x SLAMF6 -/- splenocytes after 7 days of in vitro activation with gp100 25-33 peptide and IL-2 (30 IU/ml). ( E ) Flow cytometry for activation markers (CD25, CD69, CD137) in Pmel-1 or Pmel-1 x SLAMF6 -/- splenocytes after 3 days of in vitro activation, as in ( D ). Median fluorescence intensity (MFI) is shown. ( F ) Expression of PD-1 in Pmel-1 or Pmel-1 x SLAMF6 -/- CD8+ T cells after 7 days of in vitro activation, as in ( D ). Median fluorescence intensity (MFI) is shown. ( G, H ) After 7 days of activation, Pmel-1 and Pmel-1 x SLAMF6 -/- CD8+ T cells were stained with anti-CD44 and anti-CD62L. CD8+ subpopulations were defined for each mouse strain. ( G ) One representative experiment and ( H ) summary of subpopulations identified by flow cytometry in five experiments is shown. EM, effector memory, CM, central memory. Student t-test. *, p<0.05, **, p<0.01, ***, p<0.001.

Article Snippet: Antibody , Monoclonal Syrian hamster anti mouse CD137 (17B5) , eBioscience, CA , 12-1371-82 , 0.2 μg/100 μl.

Techniques: Expressing, Flow Cytometry, Staining, In Vitro, Activation Assay, Fluorescence

Journal: eLife

Article Title: SLAMF6​ deficiency augments tumor killing and skews toward an effector phenotype revealing it as a novel T cell checkpoint

doi: 10.7554/eLife.52539

Figure Lengend Snippet:

Article Snippet: Antibody , Monoclonal Syrian hamster anti mouse CD137 (17B5) , eBioscience, CA , 12-1371-82 , 0.2 μg/100 μl.

Techniques: Generated, Immunohistochemistry, Sequencing, Recombinant, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Transformation Assay, Clonogenic Cell Survival Assay, Cell Isolation, Isolation, Software

Journal: Stem Cell Research & Therapy

Article Title: Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation

doi: 10.1186/s13287-019-1300-3

Figure Lengend Snippet: ICAM-1, CD137L, and PDL-1 are all expressed on activated feline ASCs; however, CD137L and PDL-1 do not mediate MSC-T cell adhesion. Expression of a ICAM-1, b CD137L, and c PDL-1 ligands on activated feline ASCs. Gray histogram indicated background fluorescence of unstained samples. d Percentage of remaining fluorescence intensity from CMFDA-labeled PBMCs after removal of non-adherent cells from static adhesion assay after the addition of CD137/CD137L and PD-1/PDL-1 blocking antibodies. Data is normalized to 100% on a standard MLR condition for comparability. Fluorescent images of static adhesion assay demonstrating adherent lymphocytes to ASCs from e , h non-activated MLR, f , i stimulated MLR with ConA, and g , j stimulated MLR with ConA and addition of CD137/CD137L and PD-1/PDL-1 blocking antibodies respectively. Scale bar = 400 μm. Representative flow analysis images in a – c from 3 independent experiments. Data gathered in d from 5 independent experiments

Article Snippet: In some experiments, blocking antibodies to ICAM 1 (anti-human CD54, clone MEM-111, Thermo Fisher Scientific), LFA-1 (anti-human clone R7.1, eBioscience), CD137 (anti-mouse clone 17B5, Bio X Cell), CD137L (anti-mouse clone TKS-1, Bio X Cell), PD-1 (anti-human PD-1, polyclonal goat IgG, R&D systems), or PDL-1 (anti-human PDL-1, polyclonal goat IgG, R&D systems) were added to determine which ligands mediated PBMC-ASC adhesion.

Techniques: Expressing, Fluorescence, Labeling, Cell Adhesion Assay, Blocking Assay